MR Contrast Agent Coupled to an Antisense Peptide Nucleic Acid - Cell Penetrating Peptide Conjugate
نویسندگان
چکیده
Introduction Aiming to image the presence of specific mRNAs by MRI, we have developed two intracellular MR contrast agents (CA) composed of a Gd-DOTA complex, a peptide nucleic acid (PNA) sequence and a cell penetrating peptide. One (dsRed CA) was designed to bind to mRNA of dsRed (red fluorescent protein originating from Discosoma coral) by its complementary peptide nucleic acid (PNA) sequence, whereas the second one contained a nonsense sequence with no natural counterpart.
منابع مشابه
Synthesis and cellular uptake of a MR contrast agent coupled to an antisense peptide nucleic acid--cell- penetrating peptide conjugate.
In order to image mRNA transcription by in vivo magnetic resonance imaging (MRI), two intracellular MR contrast agents were developed, which are composed of a Gd-DOTA complex, a peptide nucleic acid (PNA) sequence and a cell-penetrating peptide. One was designed to bind to mRNA of dsRed (red fluorescent protein originating from Discosoma coral) by its PNA sequence, whereas the second one contai...
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